Histological changes of the remaining live donor's kidney in rat. A light microscopic, electron microscopic and morphometric study

Twenty adult male albino rats were used in this study. The results revealed a significant increase in all the parameters measured in the remaining kidney after 2 months. Their weight was increased by 160%, the glomerular surface area increased by 140%. The proximal tubules showed an increase of their diameter by 27% and the height of their lining cells increased by 30%. With PAS technique, focal and diffuse thickenings of glomerular basement membrane were evident together with increased mesangium. However, hydropic degeneration and tubular necrosis were detected in many tubular cells after 2 months. With PAS, their basement membranes showed focal thickenings and their brush borders were thin and interrupted. The electronmicroscopic examination of the renal cortex after one month displayed focal thickenings in the glomerular basement membranes mainly at lamina densa. Marked narrowing of the glomerular capillary lumina by the proliferating endothelium and mesangium was observed. Mitochondria were swollen, vacuolated and lost their parallel alignment, endoplasmic reticulum appeared swollen. Lysosmes and pinocytotic vesicles were increased

Evaluation of the effect of oral administration of formaldehyde to albino rats

The use of formaldehyde as preservative and antimicrobial agent in milk and milk products, raises questions about its potential chronic oral toxicity. In the present work we studied the possible toxic effects of formaldehyde on the stomach of rats. Two groups of eight male and eight female rats received formaldehyde in the drinking water for a period of three months at dose level of 25 mg/kg body weight per day. Two groups of 4 male and 4 female rats served as controls and received unsupplemented drinking water liquid intake decreased in the groups receiving formaldehyde and there was yellowish discoloration of their fur. The rats were dissected and the stomach was examined after staining with Hx and E, Periodic Acid Schiff, PAS-Alcian blue and Alcian blue - Aldehyde Fuchsine. Formaldehyde induced papillary epithelial hyperplasia accompanied by hyperkeratosis, located in the limiting ridge of forestomach or in its vicinity. Glandular stomach showed focal cytolytic changes in some surface mucous cells with concomitant exfoliation and shedding in the lumen of the stomach. Dilatation of many glandular tubules was observed with vacuolization and necrosis of their lining epithelial cells i.e., mucous neck ceils, oxyntic cells and chief cells. Histochemical methods for mucousubstances demonstrated decreased reactivity of secretary products of mucous neck cells. In conclusion, the use of formaldehyde as preservative and antimicrobial agent in milk and cheese should be avoided due to its cytotoxic effects even in low doses. It is recommended to use the physical methods in milk preservation as pasteurization, instead of adding formaldehyde